EARLY DIAGNOSIS OF TUBERCULOUS PERITONITIS BY NESTED PCR AND AUTOMATED CULTURE TECHNIQUE

Complicated and delayed diagnosis of tuberculous peritonitis

BACKGROUND Peritoneal tuberculosis is very rare in European countries. However, its incidence is increasing due to the continued immigration of people from endemic areas affected by tuberculosis. CASE REPORT The authors report a case of tuberculous peritonitis in a 46-year-old male patient from North Africa. The presenting symptoms of the disease were hiccups, dyspepsia, anorexia, and weight ...

Diagnosis of Avian Mycoplasmas: A Comparison between PCR and Culture Technique

Mycoplasma gallisepticum and Mycoplasma synoviae are the causative agents of avian mycoplasmosis in commercial poultry. Among the available tools, polymerase chain reaction (PCR) and culture are confirmatory tools for the diagnosis of mycoplasmosis after the initial serological screening of suspected birds. Overall, 181 samples were analyzed, 152 (84%) and 103 (57%) of which were found positive...

Tissue PCR Diagnosis of Patients Suspicious for Tuberculous Pleurisy

Objective(s) This study planned to assess the value of PCR IS6110 assay in tissue specimens of needle pleural biopsy in patients suspicious to pleural tuberculosis. Materials and Methods Sixty eight patients with lymphocytic exudative pleural effusion underwent pleural biopsy. Tissue samples were sent for pathologic examination and PCR IS6110 assay. The results of PCR reported as positive/ nega...

Diagnosis of brucellosis by use of BACTEC blood culture and confirmation by PCR

Comparison of Betanodavirus diagnosis quality in Grouper and Sea bass by Rapid Diagnosis Kit, histopathology and Nested-PCR methods

Betanodavirus is the leading cause of viral mortality in fish worldwide and a major barrier to the spread of fisheries and aquaculture. Due to the increase in aquaculture in the Persian Gulf and the occurrence of some disease symptoms in farmed and wild fish in the region, screening of Betanodavirus-sensitive fish became important. Therefore, to prevent economic losses and reduce aquatic reserv...